Objective: To investigate in vitro antioxidant and mast cell stabilization potential Yucca gloriosa L.Methods: The aerial parts of Yucca gloriosa L. (Family- Agavaceae) were successively extracted with ethanol, 50% aqueous-ethanolic and aqueous to prepare an extract of the plant. Preliminary phytochemical tests, total phenol and flavonoid estimation were analyzed in Y. gloriosa L. extract. Furthermore, in vitro antioxidant activity was evaluated such as DPPH method, ABTS assay, superoxide assay, nitric oxide assay and lipid peroxidation inhibition assay. The extract of YGE, 50% YGE and YGA (1, 10 and 100µg/ml) was studied for peritoneal mast cell stabilization activity in rat mesenteric preparation induced by C 48/80. Result: The result revealed that YGE showed highest concentration of phenol and flavonoid compounds. Free radical scavenger activity and mast cell activity had been identified in YGE, 50%YGE and YGA extracts. The Free radical scavenger study revealed that the YGE, 50%YGE & YGA extracts of Y. gloriosa L. showed significant activity in DPPH method, Nitric oxide assay, ABTS cation decolorization assay, superoxide assay and lipid peroxidase assay. All the extracts showed ability to prevent oxidation when compare with standard markers. Upon further investigation for mast cell activity, of YGE extracts showed significant increase the number of intact cells when compared with C48/80 at the concentrations of 10 and 100 µg/mg when compare to other extracts. Conclusion: This finding provides suggested that Y. gloriosa L. is a potential candidate in herbal medicine for allergic asthma. Due to its ability to inhibit mast cell derived immediate-type I allergic reactions. It virtues further work towards the isolation of phytoconstituents from Y. gloriosa L.
Key words: Compound 48/80, Free radicals, Mast cell, Yucca gloriosa L.