Induction of Epithelial Ovarian Cancer by Implantation of 7,12-dimethylbenz(a)athracene (DMBA) Coated Silk in Rats.

    Published on:January 2019
    Journal of Young Pharmacists, 2019; 11(1):56-61
    Original Article | doi:10.5530/jyp.2019.11.12

    Ni Made Dwi Sandhiutami1,2, Wawaimuli Arozal3*, Melva Louisa3, Deni Rahmat2, Puspita Eka Wuyung4, Mokhamad Fakhrul Ulum5

    1Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.

    2Faculty of Pharmacy, University of Pancasila, Jakarta, INDONESIA.

    3Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.

    4Departement of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, INDONESIA.

    5Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, INDONESIA.


    Objective: This research aims to get the relevant animal model for epithelial ovarian cancer and ultrasonography approach to detect volume and size of ovarian cancer. Methods: Induction of ovarian cancer in rats was carried out using 7,12-Dimethylbenz [a] anthracene (DMBA) coated on silk suture and then implanted in the left ovary of female Wistar rats. Nodule were determined weekly after the first surgery by palpating the abdominal wall of rats. Utrasonography was carried out at 20th week. Rats were euthanized, ovarian organs were taken and histopathological examination was performed with haematoxylin and eosin staining. Results: Nodules in DMBA-treated rats were noted at 16th weeks post implantation and reaching a cummulative incidence of 96, 4% at 24th weeks. On ultrasound examination at the 20th week, there were significant differences in left and right ovarian size and volume. The histological type of induced tumor included: serous carcinoma cell, clear cell carcinoma, sarcoma and squamous carcinoma. Conclusion: The implanted 7,12-Dimethylbenz[a]anthracene (DMBA) coated silk suture in the ovaries rats can be used as animal model for testing therapeutics agent of ephitelial ovarian cancer and ultrasonography can determine the size and volume of tumors formed in ovary.

    Key words: 7,12-Dimethylbenz[a]anthracene, Animal model, Epithelial ovarian cancer, Ultrasonography.

    Article Download