Physicochemical Characterization, Standardization and In vitro Determination of Radical Scavenging Activity of Zereshk-e- Saghir, A Traditional Preparation, and Its Ingredients

    Published on:April 2017
    Journal of Young Pharmacists, 2017; 9(2):224-229
    Original Article | doi:10.5530/jyp.2017.9.44
    Authors:

    Zarrin Sarhadynejad1, Abbas Pardakhty2, Ali Mandegary3,4, Suleiman Afsharypuor5, Fariba Sharififar6*

    1Pharmaceutics Research Center, Institute of Neuropharmacology, Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Haft- Bagh Blvd , Kerman, IRAN.

    2Pharmaceutics Research Center, Institute of Neuropharmacology, Department of Pharmaceutics, School of Pharmacy, Haft-Bagh Blvd., Kerman, IRAN.

    3Department of Pharmacology & Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IRAN.

    4Gastroenterology and Hepatology Research Center, Afzalipour’s Hospital, Imam Highway, Kerman, IRAN.

    5Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Hezar-Jerib Ave., Isfahan, IRAN.

    6Herbal and Traditional Medicines Research Center, Department of pharmacognosy, Haft-Bagh Blvd., Kerman, IRAN.

    Abstract:

    Objective: Quality control and standardization of traditional preparations ensure their safe, pure and efficient prescription. Zereshk-e-Saghir (ZES), a folk remedy, has been recommended as a hepatoprotective agent in Traditional Persian Medicine (TPM). This study aimed at the evaluation of the physicochemical and phytochemical characterization, standardization, and in vitro antioxidant capacity determination of ZES and its ingredients. Method: Some qualitative and quantitative controls were performed like ash value, moisture content, extractable matter, phytochemical screening, TLC fingerprint and microbial contamination. Total phenolic and emodin contents of ZES were measured by spectrophotometric method to standardize this preparation. Finally in vitro radical scavenging activity of ZES and its ingredients were determined using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Result: Regarding the physicochemical evaluation, the amount of each parameter (ash value, moisture content, and extractable value) was determined as documented characteristics for ZES components. Phytochemical screening of ZES ingredients indicated the presence of tannins in all the samples and the presence of alkaloids, steroids and terpenoids in a majority of the samples. Meanwhile, ZES exhibited no pathogenic contamination. The emodin and total phenolic contents (TPC) of ZES were reported 457.0 μg emodin/100g and 381.5 mg gallic acid equivalents (GAE)/100 g of ZES, respectively. Also TPC ranged from 3607.4 to 27.9 mg GAE/ 100 g of ZES ingredients. Meanwhile, the results demonstrated that ZES could scavenge DPPH radicals (IC50value: 266.4 μg/ ml). Conclusion: The obtained results on quality control and standardization of ZES could be proposed as a monograph having standard characteristics.

    Key words: DPPH Assay, Physichochemical Characterization, Quality Control, Standardization, Traditional Medicine.

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