Background: Many research works are being carried out in preparing intracanal medicaments using herbal plants since it is safe as well as containing a better therapeutic value. The present study was designed to evaluate the anticandidal properties of the leaves of Garcinia imberti bourd in associated with the root canal infection. Method: Totally 94 root canal infected samples were collected and their previous history also recorded. Samples yielded Candida isolates were screened through drug resistant study. Multi drug-resistant Candida albicans was isolated and characterized by 18S rDNA sequencing. An antifungal activity of the extracts of the leaves of G.imberti bourd was performed by disc diffusion method. Using Silica-60 column active fraction of the methanol extract was partially purified and was analyzed by Gas Chromatography–Mass Spectrophotometry (GC-MS). Prepare intracanal medicament with methanol extract of G.imberti bourd and was tested with multidrug-resistant C.albicans (Ca-09) under in-vitro conditions using decoronated teeth. Efficacy also compared with chlorohexidine. Result: A total of 48 Candida sp. were isolated and screened for antibiotic susceptible test. Among them isolate Ca -09 showed multiple antibiotic resistant. Crude methanol fraction of G. imberti bourd leaf extract, as well as the partially purified compound of this extract, showed better anticandidal activity. 0.01g of methanol extract with 2% methyl cellulose gel showed significant reduction (63%) of C.albicans in in-vitro intracanal treatment. Conclusion: Methanol extract of Garcinia imberti bourd leaf contains glycerine, which can be used in preparing the suitable intracanal medicament for the treatment of root canal infection.
Key words: Candida albicans, Chlorohexidine, Garcinia imberti bourd, Glycerine, Intracanal medicament, Root canal infection.