Diversity of endophytic bacteria in Malaysian plants as revealed by 16S rRNA encoding gene sequence based method of bacterial identificationq

    Published on:
    Journal of Young Pharmacists, 2013; 5(3):95-97
    Short Communication | doi:10.1016/j.jyp.2013.07.001
    Authors:

    Chye Ying Loh a, Yin Yin Tan a, Rahim Rohani b, Jean-Frédéric F. Weber b, Subhash Janardhan Bhore a,*

    a Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, Malaysia.

    b Atta-ur-Rahman Research Institute of Natural Products for Drug Discovery (RiND) and Faculty of Pharmacy, MARA University of Technology (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.

    Abstract:

    Bacterial endophytes do have several potential applications in pharmacy, medicine and agricultural biotech industry. The main objective of this study was to understand types of bacterial endophytes associated with dicotyledonous (dicot) and monocotyledonous (monocot) plant species. Isolation of the endophytic bacteria was performed using surface-sterilized various tissue samples, and identification of the endophytic bacterial isolates (EBIs) was completed using 16S rRNA encoding gene sequence similarity based method. In total, 996 EBIs were isolated and identified from 1055 samples of 31 monocot and 65 dicot plant species from Peninsular Malaysia. The 996 EBIs represented 71 different types of bacterial species. Twelve (12) out of 71 species are reported as endophytes for the first time. We conclude that diverse types of bacterial endophytes are associated with dicot and monocot plants, and could be useful in pharmacy, medicine and agricultural biotechnology for various potential applications.

    Key words: Dicots, Endophytes, Monocots, Natural compounds, Bioprospecting.

     

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