Objective: To standardize Safoof-e-Binai (SB), a polyherbal Unani Powder preparation on the basis of organoleptic characters and physico-phytochemical analysis. Methods: The drugs were cleaned, dried in shade and powdered by passing through sieve # no. 80 as per the method described in UPI/ National Formulary of Unani Medicine. SB was evaluated using physicochemical tests: powder characterization, extractive value, alcohol and water-soluble matter, Ash value, LOD at 105ºC, pH and HPTLC fingerprinting. Statistical analysis used: Mean ± SEM. Results: Organoleptic characters of the formulation are Brownish green colour, characteristic odour, sweet and pungent taste and fine texture. Physicochemical parameters displayed water soluble extractive (11.23±0.04), alcohol soluble extractive (9.45±0.33), total ash (11.33±0.12), acid insoluble ash (0.29±0.02), water soluble ash (5.51±0.08), LOD at 105oC (11.40±0.04), pH of 1% and 10% solution were 7.33±0.33 and 7.66±0.33 respectively. Phytochemical qualitative analysis displayed presence of alkaloids, tannins, flavanoids, terpenoids, carbohydrates, volatile oil. HPTLC finger-printing data was also set in. Conclusion: The standardization of this formulation was done and the data obtained would be used as a standard for future reference.
Key words: Physicochemical, Poly-herbal, Formulation, Standardization, Safoof-e- Binai, Unani Medicine. .